Good cells are the guarantee of the success of the experiment, so the question is, which global cell is stronger? To the leading expert ATCC.
Founded in 1925, ATCC is a global, non-profit biological standards resource center governed by a board of 14 biochemical and medical industry associations in the United States. It has the world's largest and richest variety of microorganisms, cell lines and recombinant DNA raw materials, and is also recognized by researchers as a supplier of world-class and reliable research materials.
Today, Xiaobian has summarized some problems about ATCC cell transportation, storage, and culture. Interested partners walk by and pass by, don't miss it!
Cell transport and cell post-treatment
ATCC cells are usually stored in freeze-storage tubes, transported on dry ice, or transported at room temperature in culture bottles. However, for Chinese customers, due to the slightly longer transportation time, dry ice transportation is used.
Upon receipt of the frozen cells, it is important to thaw them quickly to resuscitate them immediately and remove DMSO, and then place them in the medium. If this is not possible, the cells need to be stored in liquid nitrogen (below -130 ° C). Do not store frozen cells at temperatures higher than -130 ° C, which will rapidly reduce cell survival.
How to obtain cell instructions
In order to respond to the call for environmental protection, and to ensure that information is updated quickly. ATCC cell lines were shipped without a paper manual.
The ATCC manual has detailed procedures for cell recovery and passage. In addition to the specification, there is another important document - COA (Certificate of Analysis). The COA contains information about the specific batch, the number of cells per tube, the amount of media recommended for resuscitation and other important information.
These two documents need to be downloaded from the ATCC website.
If you encounter downloading problems, please contact us in time!
Selection of medium
To resuscitate the cells, it is necessary to prepare a suitable medium, serum and additives required for cell growth. While most cell lines can be grown in more than one medium, the properties of the cells change when the medium is changed. In addition, some media such as DMEM, the manufacturers provide products although the name is the same, but the composition will be different.
In order to ensure maximum cell recovery and maintain cell characteristics, it is recommended to purchase ATCC media at the time of cell purchase (see product information sheet and ATCC website).
How do I buy ATCC media?
Beijing Zhongyuan company agent ATCC full line of products. You can of course purchase ATCC media from us. Due to the problem of transportation time, please purchase the supporting media when you buy the cells to avoid the embahhassment of no media when the cells ahhive.
ATCC is a global, non-profit biological resource center that provides biological products, technical support and training to corporations, governments and scientific institutions worldwide. It takes the acquisition, classification, summary, improvement and wide dissemination of comprehensive knowledge in the field of life science as its mission, with a view to playing a certain role in promoting the forefront, legalization and practicality of biological raw materials, biological information, biotechnology, intellectual property rights, norms and other related fields.
Beijing Zhongyuan Company is an authorized distributor of ATCC, to help you easily order ATCC products, enjoy professional and intimate service.